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Lexicon

Sleep

DSIP

The delta-sleep-inducing peptide, meant to promote deep sleep, with an interesting but mixed evidence record.

2 min read 1 sources Calculator Updated May 2026
Class
Neuropeptide (sleep)
Use
Subcutaneous (in mcg)
Half-life
short
Status
Older data, mixed

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Equipment

Equipment you need

For reconstitution and injection you need a bit of basic gear. Here are solid, cheap options:

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What is DSIP?

DSIP (delta-sleep-inducing peptide) is an endogenous neuropeptide discovered in the 1970s and named for its ability to induce deep sleep (delta sleep) in animal models. It is used with the goal of deepening sleep and stabilizing the day-night rhythm.

How it works

The exact mechanism of DSIP is not fully understood. Described are effects on the sleep rhythm, on brain messengers, on hormone levels and on the stress response. It acts more as a modulator than sedating like a classic sleeping pill.

What you need

  • The DSIP vial (lyophilized powder).
  • Bacteriostatic water, U-100 insulin syringes, alcohol swabs.

Reconstitution

Use the calculator above. A 5mg vial with 2ml water gives 2500mcg per ml, 200mcg is then 8 units on the U-100 syringe. Just add the water to the vial and let it dissolve.

Dosing

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Injection

Subcutaneous into the subcutaneous fat, in the evening. Disinfect the site, pinch a skin fold, rotate sites.

What the research shows

A detailed review (Graf & Kastin, 1984) summarized DSIP research: effects on deep sleep in several species and in humans, plus effects on hormones and stress. But clinical efficacy in sleep disorders was mixed, a clear therapeutic benefit could not be pinned down.

Side effects

  • Generally well tolerated, mainly local reactions at the injection site.
  • Occasionally headache, dizziness.
  • The effect on sleep varies greatly between individuals.

Practice

  • Use in the evening, keep expectations realistic, the data is mixed.
  • Sleep hygiene beats any peptide, DSIP is at most an add-on.

Storage

Before mixing the powder is robust, normal room temperature (around 20 to 25 degrees) is fine, ideally kept in the dark. After mixing, keep in the fridge (2 to 8 degrees), do not freeze. It stays good there for at least 8 to 10 weeks with barely any loss of potency, in practice considerably longer. Swirl or shake to mix, both are fine.

Evidence

Sources

  1. 1 Delta-sleep-inducing peptide (DSIP): review of sleep and effects. Graf & Kastin, Neuroscience & Biobehavioral Reviews, 1984

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