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Test/Tren Long 300

200mg testosterone enanthate and 100mg trenbolone enanthate per ml: a testosterone base plus trenbolone in long esters, two injections per week but the full tren profile.

3 min read 12 sources

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Glass vial of Test/Tren Long 300 with TPD label on a white podium with a light blue ring of light in front of a bright lab background, symbolic image
No study of its own · not approved

Summary

At a glance

Sources checked on 26 Oct 2026

Phase 3 on testosterone enanthate
7 trials

on the registry as injection, none on the blend or on trenbolone, as of 26 Oct 202610

Binding at the androgen receptor
~3 × testosterone

Trenbolone according to a review, data almost only from animal studies2

Fat-free mass, highest testosterone level
+7.9 kg

20 weeks, highest of five dose levels of testosterone enanthate (the two below +5.2 and +3.4 kg)1

With high blood pressure
51 %

Current users on 24-hour monitoring, compared with 17 percent without steroids4

Pumping function of the heart
52 %

Long-term users, compared with 63 percent in non-users5

  • Test/Tren Long 300 is a ready-made oil of testosterone enanthate and trenbolone enanthate in a 2 to 1 ratio. Both esters are long, testosterone enanthate is released from the oil over days.6
  • According to a review trenbolone binds the androgen receptor about three times as strongly as testosterone and acts less androgenic and less estrogenic in tissue. The data come almost only from animal studies.2
  • There is no study on the blend and no registered human study on trenbolone. Testosterone enanthate increased fat-free mass depending on the amount while HDL cholesterol fell.101
  • Users had higher blood pressure and a stiffer aorta. Long-term users had weaker heart pumping function and more plaque in the coronary arteries.45
  • According to ChEMBL testosterone enanthate has been approved since 1953, trenbolone is not approved for humans. Everything here is the state of research, not a recommendation.1112
Profile

Updated October 2026

Class
Anabolic steroid

oil blend of two long esters

Contents
200mg testosterone enanthate + 100mg trenbolone enanthate per ml (300mg/ml)
Use
Intramuscular, ready-to-use oil

10 ml

Frequency
2x per week
WADA
Banned

category S1

Sizes
10 ml

1 size in the shop

Contents 14 sections
  1. At a glance
  2. What is Test/Tren Long 300?
  3. How it works
  4. Use and dosing
  5. Injection
  6. What the research shows
  7. Research status
  8. Side effects
  9. Storage
  10. PCT / after coming off
  11. Related compounds
  12. FAQ
  13. Glossary
  14. Sources

What is Test/Tren Long 300?

Test/Tren Long 300 is a ready-made oil solution with 200mg testosterone enanthate and 100mg trenbolone enanthate per ml, 300mg/ml in total. One vial holds 10 ml and needs no mixing. "Long" refers to the long enanthate esters of both compounds.

The 2:1 ratio means there is always twice as much testosterone as trenbolone in play, so the testosterone base is built in. Trenbolone comes from livestock use and was never approved for humans. Because of its side-effect profile the blend is nothing for beginners.

This information is for research and educational purposes only. No medical advice.

Comparison

Testosterone and trenbolone in four oils

Which compounds each oil contains, as stated in the TPD lexicon. Tap a card to open its entry.

Short esters Test/Tren Short 150 Testosterone Trenbolone Boldenone 2 of 3 compounds
Long esters Test/Tren Long 300 Testosterone Trenbolone Boldenone 2 of 3 compounds
Long esters Test/Tren/EQ Mix 400 Testosterone Trenbolone Boldenone 3 of 3 compounds Single compound Trenbolone Enanthate Testosterone Trenbolone Boldenone 1 of 3 compounds

Simplified: the same compounds do not mean the same amount per ml. Every extra compound brings its own side effects.

How it works

Both compounds bind the androgen receptor in muscle and drive protein synthesis and strength. Trenbolone binds much more strongly than testosterone, does not aromatize to estradiol and holds little water. It also acts on the progesterone receptor, though, which can favor prolactin effects.

Testosterone keeps estradiol and DHT in a normal range, which supports libido and wellbeing. Both enanthate esters have a half-life of several days, so levels stay stable with two doses per week. On the flip side, side effects take one to two weeks to fade after stopping.

Where it acts

One receptor, several organs

Tap a point or a card. Grey = effect seen in trials.

  1. Hypothalamus and pituitary

    LH and FSH suppressed as long as testosterone was raised6

    Observed in trials
  2. Testes

    Sperm production suppressed: no sperm in 65 percent after 6 months, recovery within months after stopping7

    Observed in trials
  3. Blood

    Haemoglobin and haematocrit rose with the testosterone dose, more in older men8

    Observed in trials
  4. Heart

    Pumping function lower in long-term users (52 vs 63 percent), more coronary plaque5

    Observed in trials
  5. Skeletal muscle

    More muscle and bone mass under trenbolone in various animal species2

    Observed in trials
  6. Fat tissue

    Fat mass fell as testosterone levels rose1

    Observed in trials
  7. Skin

    Acne in 28 of 100 users, breast tissue growth in 199

    Observed in trials

Targets in the ChEMBL database11

Compound CHEMBL1200335: one recorded mechanism, agonist with direct binding to the human receptor.

Androgen receptor
Gene
AR
UniProt
P10275
ChEMBL
CHEMBL1871
Action
Agonist, direct binding
Translucent glass muscle fibres in a steady stream of blue steroid molecules coming from a golden oil droplet
Long esters release the compounds evenly from the oil depot. Symbolic image.

Use and dosing

Dosing

Dosing, titration and syringe

Loading the dosing information …

  • Getting started and stepping up, drawn as a staircase
  • Units on the U-100 syringe for every step
  • How long one vial lasts
Glass vial of Test/Tren Long 300 with clear, slightly yellowish oil and a grey stopper, next to it a closed syringe with its cap on a light table
Ready-to-use oil, no mixing needed. Symbolic image.

Injection

Intramuscular into the glute, thigh or shoulder, with a thick needle to draw up and a thin one (23G to 25G) to inject. Disinfect the stopper and the site first and rotate sites regularly. Aspirating first and injecting slowly lowers the risk of tren cough, a brief coughing fit right after the injection.

Both esters are long, but trenbolone irritates subcutaneous tissue more. Subcutaneous use therefore only at very small volumes and only if well tolerated.

What the research shows

For testosterone a dose-response study shows that fat-free mass and strength rise with the dose while HDL falls in step (Bhasin, 2001). Trenbolone is described as a very potent anabolic with reduced androgenic and estrogenic activity; the evidence comes almost entirely from animal studies (Yarrow, 2010). There is no study of the fixed combination.

Reviews and observational studies of steroid users show shifted lipids (Achar, 2010), lastingly higher blood pressure with a stiffer aorta (Rasmussen, 2018) plus, with long-term use, weaker heart pumping function and more coronary plaque (Baggish, 2017).

Study results

What the trials measured

Interactive: switch tabs, tap points, show or hide arms. Arms are named relative to each other, no dosing advice.

Fat-free mass after 20 weeks1

Gain in kg in the three higher of five dose levels of testosterone enanthate, the body's own production was switched off with medication

Trial with five dose levels1 61 young men, 20 weeks
Level 3 of 5 +3.4 kg
Level 4 of 5 +5.2 kg
Level 5 of 5 (highest, 600 mg per week) +7.9 kg

The two lowest levels are not shown here. Strength, haemoglobin and IGF-1 rose with the testosterone level while fat mass and HDL cholesterol fell. Trial with testosterone alone.

High blood pressure in users4

Share with systolic hypertension on 24-hour monitoring, strength athletes

Cross-sectional study, 100 men4 37 current users, 30 controls
Current users 51%
Strength athletes without steroids 17%

Systolic 24-hour average 132 vs 124 mmHg. Aortic distensibility was 21 percent lower in current and in former users than in controls, so the aorta was stiffer.

Pumping function of the heart5

Left ventricular ejection fraction in percent, experienced strength athletes aged 34 to 54

Users vs non-users5 86 and 54 men
Long-term users 52%
Non-users 63%
Users only: on steroids or off5 58 and 28 men
Users, currently on steroids 49%
Users, currently off 58%

Cross-sectional study, users with at least 2 years of total use. Coronary plaque had a median of 3 vs 0, the longer the total use, the more plaque.

Incubator shelves with petri dishes, next to them a glass hourglass with slowly running sand and a frosted glass kidney
For trenbolone there are almost only animal data. Symbolic image.

Research status

Where the research stands

There is no study on the blend and the registry finds none on trenbolone. Shown are registered trials with testosterone enanthate given by injection. As of 26 Oct 2026. 10

  1. Preclinical Trenbolone: more muscle and bone mass, less fat in various animal species
  2. Phase 1 9 trials on components, 8 completed, 1 stopped early
  3. Phase 2 13 trials on components, 8 completed, 1 stopped early
  4. Phase 3 7 trials on components, 6 completed
  5. Approval not yet
586 participants in phase 3 Planned figures for running trials Not approved as a blend. According to ChEMBL testosterone enanthate has been approved since 1953, trenbolone has no approval for humans.11
Show all 23 phase 2, 3 and 4 trials on a timelineHide timeline 17 completed 5 running 1 stopped early 0 with results
Phase 2
Phase 3
Phase 4

Bar: start to primary endpoint (planned for running trials). Green = completed, blue = progress until 26 Oct 2026, grey = stopped early. Phase 1: 9 trials on safety and pharmacokinetics, not shown as bars.

Side effects

  • Prolactin: trenbolone can cause prolactin effects, with libido and erection problems. Add prolactin to the bloodwork.
  • Lipids: HDL drops sharply and LDL rises. Trenbolone is considered especially unfavorable here.
  • Blood pressure and heart: blood pressure and resting heart rate go up, endurance and breathing under load suffer. Measure regularly.
  • Sleep: trouble falling asleep, restless sleep and night sweats are typical.
  • Mood: irritability, restlessness and aggression are reported often.
  • Estrogen and blood count: the testosterone share aromatizes, hematocrit rises.
  • Hormone axis: your own production is strongly suppressed, acne and hair loss if predisposed are common.

Blood pressure, lipids, prolactin and hematocrit should be checked before, during and after a cycle.

Tolerability

Frequency in the HAARLEM study

Out of 100 users, how many had the event. One cohort without a comparison group, participants chose compounds and amounts themselves.

100 of 100 All 100 users
Event All 100 users
At least one side effect9 100%
Lower libido after the cycle9 58%
Fluid retention during the cycle9 56%
Agitation during the cycle9 36%
Acne9 28%
Breast tissue growth (gynecomastia)9 19%

Four participants had a serious event: heart failure, pancreatitis, suicidal thoughts and a flare of ulcerative colitis. One year after the start the observed effects were back at baseline. Effects typical of trenbolone such as sleep problems were not reported separately.9

Storage

Store at room temperature, in the dark and in the original packaging, out of reach of children. Do not put it in the fridge, because cold makes the compounds crystallize out of the oil. If you see crystals, warm the vial in your hand or in warm water and swirl until the solution is clear again. Disinfect the stopper before every draw.

Four glass vials with golden oil and grey caps on a white shelf, next to them a folded light blue cloth
Store at room temperature, dark and closed, not in the fridge. Symbolic image.

Related

Related compounds

Same compounds: all in the TPD lexicon.

Test/Tren Long 300
Testosterone Enanthate Component, single entry in the lexicon Trenbolone Enanthate Component, single entry in the lexicon Test/Tren Short 150 Same compounds with short esters Test/Tren/EQ Mix 400 Plus boldenone undecylenate Trenbolone Acetate Trenbolone with a short ester Tren/Test/Mast Short 150 Short esters plus drostanolone Blend 500 (Tren E + Mast E) Trenbolone enanthate plus drostanolone enanthate

FAQ

Common questions about Test/Tren Long 300

Short answers, each backed by the sources below.

What is in Test/Tren Long 300?

Testosterone enanthate and trenbolone enanthate in a 2 to 1 ratio, ready to use in oil. Long stands for the long enanthate esters. Because the shares are fixed, any change in amount always changes both compounds.1112

What is special about trenbolone?

According to a review trenbolone binds the androgen receptor about three times as strongly as testosterone. In tissue testosterone is partly converted into DHT and estradiol while trenbolone is converted into weaker androgens. That is why it acts less androgenic and less estrogenic in tissue. In various animal species it increased muscle and bone mass and lowered body fat. There are no controlled trials in humans.2

Are there studies on this blend?

No. As of 26 October 2026 ClinicalTrials.gov lists no study on the blend and none on trenbolone. For testosterone enanthate by injection it lists 9 phase 1, 13 phase 2, 7 phase 3 and 3 phase 4 trials.10

Does the effect of the testosterone share depend on the amount?

Yes, clearly. In a trial with five dose levels of testosterone enanthate over 20 weeks (the body's own production was switched off with medication) fat-free mass rose by 3.4, 5.2 and 7.9 kg in the three higher levels. Leg strength, muscle volume and haemoglobin rose with the testosterone level while fat mass and HDL cholesterol fell. Mood and PSA did not change clearly at any level.1

What do studies show on heart and circulation?

In current users the 24-hour systolic blood pressure averaged 132 vs 124 mmHg and 51 percent had high blood pressure compared with 17 percent without steroids. Former users also had a stiffer aorta. In another study long-term users pumped less blood per heartbeat (ejection fraction 52 vs 63 percent) and had more plaque in the coronary arteries. A review of 49 studies with 1,467 athletes also describes unfavourable blood lipids and a thickened heart wall.453

What happens to the body's own hormone production?

Testosterone from outside suppresses the hormone axis. After one injection of testosterone enanthate LH and FSH stayed suppressed as long as the level was raised. In the WHO study with weekly injections a cumulative 65 percent had no detectable sperm after 6 months. After stopping it took a median of 3.7 months until at least 20 million sperm per ml returned and 6.7 months until baseline. How long recovery takes after this blend has not been studied.67

Which side effects do users report?

The HAARLEM study followed 100 men through a self-chosen cycle and one year. All reported at least one side effect, most often fluid retention (56 percent) and agitation (36 percent) during the cycle and lower libido afterwards (58 percent). Acne occurred in 28 percent and breast tissue growth in 19 percent. Four had a serious event. After one year the observed effects were back at baseline. Effects typical of trenbolone such as sleep problems or prolactin effects were not reported separately there.9

Glossary

Terms explained briefly

Dotted words in the text open these explanations directly.

19-nor steroid
Testosterone derivative without the carbon atom at position 19. Nandrolone and trenbolone belong to this group.
Androgen receptor
Protein inside the cell that testosterone and related compounds bind to. Genes are then read, for example for muscle building.
Cohort study
Observational study that follows a group over time. Participants choose compounds and amounts themselves, there is no random allocation.
Ester
An attached fatty acid chain. It makes the compound oil-soluble and sets how slowly it is released from the depot. It is split off in the blood.
Estradiol
The main oestrogen. In men it is also made from testosterone and rises with the testosterone level.
Fat-free mass
Body weight without fat: muscle, bone, organs and water.
Haematocrit
Share of red blood cells in the blood. If it rises a lot, the blood gets thicker.
HDL cholesterol
The so-called good cholesterol. It carries fat from the vessels back to the liver.
Hormone axis (HPTA)
Hypothalamus, pituitary and testes together control the body's own testosterone production. Testosterone from outside suppresses this axis.
Progesterone receptor
Receptor for the hormone progesterone. Some anabolic steroids also act here, not only at the androgen receptor.
Prolactin
Pituitary hormone. If it is raised, libido and erection can suffer.
Pumping function (ejection fraction)
Share of blood the left ventricle pushes out with each beat. A lower value means weaker pumping.

Practice

PCT / after coming off

Anabolic substances suppress the body's own testosterone production in the research context. Anyone who comes off a cycle (rather than running it continuously) typically uses a post-cycle therapy (PCT) to restart the natural axis (HPTA).

The standard scheme in the research/literature context combines a SERM (tamoxifen and/or clomifene, alternatively enclomiphene) over roughly four weeks, optionally preceded by HCG to re-sensitise the testes before the SERM block. An aromatase inhibitor (anastrozole/exemestane) belongs more to on-cycle estrogen control than to the PCT itself.

Tip: order the PCT items together with the cycle so they are already on hand when you come off.

This information serves research and educational purposes only. Not medical advice.

Tamoxifen (Nolvadex) SERM, core of PCT View Clomid (Clomifen) SERM, core of PCT View Enclomiphene (Androxal) SERM alternative View HCG testicular restart before PCT View Arimidex (Anastrozole) aromatase inhibitor, on-cycle View Aromasin (Exemestane) aromatase inhibitor, on-cycle View

Evidence

Sources

Show all 12 sourcesHide sources 9 Studies1 Trial registry2 Database checked 26 Oct 2026

Studies

  1. Dose response in young men, five levels over 20 weeks

    Bhasin et al., American Journal of Physiology: Endocrinology and Metabolism, 2001

    DOI 10.1152/ajpendo.2001.281.6.E1172 PMID 11701431
    Checked 26 Oct 2026 via doi.org, Crossref, PubMed
  2. Review on trenbolone: binding, metabolism and tissues

    Yarrow et al., Steroids, 2010

    DOI 10.1016/j.steroids.2010.01.019 PMID 20138077
    Checked 26 Oct 2026 via doi.org, Crossref, PubMed
  3. Review: heart and metabolism under anabolic steroids, 49 studies

    Achar et al., American Journal of Cardiology, 2010

    DOI 10.1016/j.amjcard.2010.05.013 PMID 20816133
    Checked 26 Oct 2026 via doi.org, Crossref, PubMed
  4. Blood pressure and aortic stiffness in current and former users

    Rasmussen et al., Journal of Hypertension, 2018

    DOI 10.1097/hjh.0000000000001546 PMID 28863033
    Checked 26 Oct 2026 via doi.org, Crossref, PubMed
  5. Heart function and coronary plaque after long-term use

    Baggish et al., Circulation, 2017

    DOI 10.1161/CIRCULATIONAHA.116.026945 PMID 28533317
    Checked 26 Oct 2026 via doi.org, Crossref, PubMed
  6. Level over time after one injection of testosterone enanthate

    Schürmeyer and Nieschlag, International Journal of Andrology, 1984

    DOI 10.1111/j.1365-2605.1984.tb00775.x PMID 6434435
    Checked 26 Oct 2026 via doi.org, Crossref, PubMed
  7. WHO study: sperm production under weekly injections

    WHO Task Force on Methods for the Regulation of Male Fertility, The Lancet, 1990

    DOI 10.1016/0140-6736(90)92416-F PMID 1977002
    Checked 26 Oct 2026 via doi.org, Crossref, PubMed (Titel und Seiten, Datensatz ohne DOI)
  8. Red blood cell production (haemoglobin, haematocrit) by testosterone dose

    Coviello et al., Journal of Clinical Endocrinology and Metabolism, 2008

    DOI 10.1210/jc.2007-1692 PMID 18160461
    Checked 26 Oct 2026 via doi.org, Crossref, PubMed
  9. HAARLEM study: 100 users followed for one year

    Smit et al., Scandinavian Journal of Medicine and Science in Sports, 2020

    DOI 10.1111/sms.13843 PMID 33038020
    Checked 26 Oct 2026 via doi.org, Crossref, PubMed (Heft 2021, online 2020)

Trial registry

  1. Trials on testosterone enanthate on ClinicalTrials.gov

    ClinicalTrials.gov, U.S. National Library of Medicine

    Checked 26 Oct 2026 via ClinicalTrials.gov API v2

Database

  1. Testosterone enanthate in ChEMBL: mechanism and approval

    ChEMBL, EMBL-EBI (CHEMBL1200335)

    Checked 26 Oct 2026 via ChEMBL
  2. Trenbolone acetate in ChEMBL: not approved, no recorded mechanism

    ChEMBL, EMBL-EBI (CHEMBL1698011)

    Checked 26 Oct 2026 via ChEMBL

In the shop

Test/Tren Long 300 in the catalog

Sizes, prices and shipping times are on the product page.

To the product