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Test/Tren/EQ Mix 400

Three long esters in one oil: 150mg testosterone enanthate, 150mg boldenone undecylenate and 100mg trenbolone enanthate per ml, a blend with trenbolone's harsh profile.

4 min read 13 sources

To the product To the sources
Glass vial of Test/Tren/EQ Mix 400 with TPD label on a white podium with a light blue ring of light in front of a bright lab background, symbolic image
No study of its own · not approved

Summary

At a glance

Sources checked on 26 Oct 2026

Phase 3 on testosterone enanthate
7 trials

on the registry as injection, none on the blend, on trenbolone or on boldenone, as of 26 Oct 202610

Binding at the androgen receptor
~3 × testosterone

Trenbolone according to a review, data almost only from animal studies2

Bench press in 10 weeks
+22 kg

Testosterone enanthate with strength training, 43 healthy men, +9 kg without training1

At least one side effect
100 %

HAARLEM cohort with 100 users, 4 of them with a serious event5

HDL cholesterol
-60.2 %

Strength athletes, several steroids at once over 8 or 14 weeks, not recovered after a 6-week break6

  • Test/Tren/EQ Mix 400 is a ready-made oil of testosterone enanthate, boldenone undecylenate and trenbolone enanthate in a 3 to 3 to 2 ratio. All three esters are long.111213
  • There is no study on the blend and no registered human study on trenbolone or boldenone. The figures here come from studies on testosterone alone, animal data on trenbolone and observations in users.10
  • According to a review trenbolone binds the androgen receptor about three times as strongly as testosterone and acts less androgenic and less estrogenic in tissue.2
  • Testosterone enanthate increased muscle size and strength, most of all with training. Haemoglobin and haematocrit rose with the amount.13
  • In a cohort of 100 users every one had at least one side effect, four had a serious event. HDL cholesterol fell by about 60 percent under several steroids. Everything here is the state of research, not a recommendation.56
Profile

Updated October 2026

Class
Anabolic steroid

oil blend of three long esters

Contents
150mg testosterone enanthate + 150mg boldenone undecylenate + 100mg trenbolone enanthate per ml (400mg/ml)
Use
Intramuscular, ready-to-use oil

10 ml

Frequency
2x per week
WADA
Banned

category S1

Sizes
10 ml

1 size in the shop

Contents 14 sections
  1. At a glance
  2. What is Test/Tren/EQ Mix 400?
  3. How it works
  4. Use and dosing
  5. Injection
  6. What the research shows
  7. Research status
  8. Side effects
  9. Storage
  10. PCT / after coming off
  11. Related compounds
  12. FAQ
  13. Glossary
  14. Sources

What is Test/Tren/EQ Mix 400?

Test/Tren/EQ Mix 400 is a ready-made oil solution of three compounds: 150mg testosterone enanthate, 150mg boldenone undecylenate (Equipoise) and 100mg trenbolone enanthate per ml, 400mg/ml in total. One vial holds 10 ml and needs no mixing. All three esters are long, so two injections per week are enough.

Testosterone provides the base, boldenone a slow second building compound and trenbolone the strongest but also most demanding part. Trenbolone and boldenone come from veterinary medicine and were never approved for humans. Not a beginner blend: the tren share sets the side-effect profile.

This information is for research and educational purposes only. No medical advice.

Comparison

One, two or three compounds

Which compounds each oil contains, as stated in the TPD lexicon. Tap a card to open its entry.

1 compound Trenbolone Enanthate Testosterone Boldenone Trenbolone 1 of 3 compounds 2 compounds Test/EQ Mix 400 Testosterone Boldenone Trenbolone 2 of 3 compounds 2 compounds Test/Tren Long 300 Testosterone Boldenone Trenbolone 2 of 3 compounds
3 compounds Test/Tren/EQ Mix 400 Testosterone Boldenone Trenbolone 3 of 3 compounds

Simplified: the same compounds do not mean the same amount per ml. Every extra compound brings its own side effects.

How it works

All three compounds activate the androgen receptor. Trenbolone binds it much more strongly than testosterone, is not aromatized to estradiol and also acts on the progesterone receptor, which can favor prolactin effects. Boldenone aromatizes weakly and stimulates red blood cell production, while testosterone aromatizes normally.

Half-lives are about 4 to 5 days (enanthate) and about two weeks (undecylenate). The full effect builds over several weeks. In turn, side effects also fade only slowly after stopping.

Where it acts

One receptor, several organs

Tap a point or a card. Grey = effect seen in trials.

  1. Hypothalamus and pituitary

    LH and FSH suppressed as long as testosterone was raised8

    Observed in trials
  2. Testes

    Sperm production suppressed: no sperm in 65 percent after 6 months, recovery within months after stopping9

    Observed in trials
  3. Blood

    Haemoglobin and haematocrit rose with the testosterone dose, more in older men3

    Observed in trials
  4. Liver and blood lipids

    HDL cholesterol fell by about 60 percent under several anabolic steroids at once6

    Observed in trials
  5. Heart

    Pumping function lower in long-term users (52 vs 63 percent), more coronary plaque7

    Observed in trials
  6. Skeletal muscle

    Muscle size in arms and legs and strength increased, most with training1

    Observed in trials
  7. Skin

    Acne in 28 of 100 users, breast tissue growth in 195

    Observed in trials

Targets in the ChEMBL database11

Compound CHEMBL1200335: one recorded mechanism, agonist with direct binding to the human receptor.

Androgen receptor
Gene
AR
UniProt
P10275
ChEMBL
CHEMBL1871
Action
Agonist, direct binding
Translucent glass muscle fibres with docking sites where different blue steroid molecules dock, a sapphire one glows brightest
Three compounds at the androgen receptor, trenbolone binds most strongly. Symbolic image.

Use and dosing

Dosing

Dosing, titration and syringe

Loading the dosing information …

  • Getting started and stepping up, drawn as a staircase
  • Units on the U-100 syringe for every step
  • How long one vial lasts
Glass vial of Test/Tren/EQ Mix 400 with clear, slightly yellowish oil and a grey stopper, next to it a closed syringe with its cap on a light table
Ready-to-use oil, no mixing needed. Symbolic image.

Injection

Intramuscular into the glute, thigh or shoulder, with a thick needle to draw up and a thin one (23G to 25G) to inject. Disinfect the stopper and the site first and rotate sites regularly. Injecting slowly and aspirating first helps against the so-called tren cough, a brief coughing fit right after the injection.

The esters are long, but trenbolone irritates subcutaneous tissue more. Subcutaneous use therefore only at very small volumes and only if well tolerated, otherwise intramuscular.

What the research shows

For testosterone, gains in muscle mass and strength at supraphysiologic doses are well documented (Bhasin, 1996), as is the dose-dependent rise in hemoglobin and hematocrit (Coviello, 2008). Trenbolone is described as a very potent anabolic with reduced androgenic and estrogenic activity, but the data come almost entirely from animal studies (Yarrow, 2010). There are no controlled human studies on boldenone.

Reviews of anabolic steroids show shifted lipids, higher blood pressure plus changes to heart wall and rhythm (Achar, 2010). In a prospective cohort of 100 users every one had at least one side effect and four had a serious event (Smit, 2020). No study exists for the fixed three-way combination.

Study results

What the trials measured

Interactive: switch tabs, tap points, show or hide arms. Arms are named relative to each other, no dosing advice.

Strength gain in 10 weeks1

Healthy men, weekly injection of testosterone enanthate or placebo, gain in kg

Bench press1 43 men, 10 weeks
Placebo without training -1 kg
Testosterone without training +9 kg
Testosterone with training +22 kg
Squat1 43 men, 10 weeks
Placebo without training +3 kg
Testosterone without training +16 kg
Testosterone with training +38 kg

Trial with testosterone enanthate alone, not with the blend. The abstract gives no figures for the placebo with training group. Mood and behaviour did not change in any group.

Blood lipids under several anabolic steroids6

Change in percent, 19 strength athletes, self-chosen cycle over 8 or 14 weeks

HDL cholesterol6
Several anabolic steroids at once -60.2%
Apolipoprotein A16
Several anabolic steroids at once -49.6%
Apolipoprotein B6
Several anabolic steroids at once +37.5%
Lipoprotein (a)6
Several anabolic steroids at once -83.1%

Percentages calculated from the mean values in the abstract: HDL from 1.08 to 0.43 mmol/l, apo-A1 from 1.41 to 0.71 g/l, apo-B from 0.96 to 1.32 g/l, Lp(a) from 189 to 32 U/l. Six weeks after stopping the values had not yet recovered. Nandrolone decanoate alone did not change blood lipids in the second part of the study.

Pumping function of the heart7

Left ventricular ejection fraction in percent, experienced strength athletes aged 34 to 54

Users vs non-users7 86 and 54 men
Long-term users 52%
Non-users 63%
Users only: on steroids or off7 58 and 28 men
Users, currently on steroids 49%
Users, currently off 58%

Cross-sectional study, users with at least 2 years of total use. Coronary plaque had a median of 3 vs 0, the longer the total use, the more plaque.

Close-up of sealed test tubes reflecting a glowing glass heart and a glass muscle sculpture
There are studies on testosterone, for trenbolone almost only animal data. Symbolic image.

Research status

Where the research stands

There is no study on the blend and the registry finds none on trenbolone or boldenone. Shown are registered trials with testosterone enanthate given by injection. As of 26 Oct 2026. 10

  1. Preclinical Trenbolone: more muscle and bone mass, less fat in various animal species
  2. Phase 1 9 trials on components, 8 completed, 1 stopped early
  3. Phase 2 13 trials on components, 8 completed, 1 stopped early
  4. Phase 3 7 trials on components, 6 completed
  5. Approval not yet
586 participants in phase 3 Planned figures for running trials Not approved as a blend. According to ChEMBL testosterone enanthate has been approved since 1953, boldenone undecylenate and trenbolone are not.11
Show all 23 phase 2, 3 and 4 trials on a timelineHide timeline 17 completed 5 running 1 stopped early 0 with results
Phase 2
Phase 3
Phase 4

Bar: start to primary endpoint (planned for running trials). Green = completed, blue = progress until 26 Oct 2026, grey = stopped early. Phase 1: 9 trials on safety and pharmacokinetics, not shown as bars.

Side effects

  • Prolactin: through its progestogenic action trenbolone can cause prolactin effects, with libido and erection problems. Add prolactin to the bloodwork.
  • Lipids: HDL drops markedly and LDL rises. Trenbolone is considered especially unfavorable here.
  • Blood pressure and heart: blood pressure and resting heart rate go up, endurance drops noticeably. Measure regularly.
  • Sleep: trouble falling asleep, restless sleep and night sweats are typical of the tren share.
  • Mood: irritability, restlessness and aggression are reported often.
  • Blood count: testosterone and boldenone raise hematocrit and hemoglobin, which increases the risk of thrombosis.
  • Other: suppressed hormone axis, acne, hair loss if predisposed, water retention from the testosterone share.

Because the esters are long, these effects cannot be switched off quickly. That is why a low start and bloodwork before, during and after a cycle matter.

Tolerability

Frequency in the HAARLEM study

Out of 100 users, how many had the event. One cohort without a comparison group, participants chose compounds and amounts themselves.

100 of 100 All 100 users
Event All 100 users
At least one side effect5 100%
Lower libido after the cycle5 58%
Fluid retention during the cycle5 56%
Agitation during the cycle5 36%
Acne5 28%
Breast tissue growth (gynecomastia)5 19%

Four participants had a serious event: heart failure, pancreatitis, suicidal thoughts and a flare of ulcerative colitis. One year after the start the observed effects were back at baseline. Effects typical of trenbolone such as sleep problems were not reported separately.5

Storage

Store at room temperature, in the dark and in the original packaging, out of reach of children. Do not put it in the fridge, because cold makes the compounds crystallize out of the oil. If you see crystals, warm the vial in your hand or in warm water and swirl until the solution is clear again. Disinfect the stopper before every draw.

Four glass vials with golden oil and grey caps on a white shelf, next to them a folded light blue cloth
Store at room temperature, dark and closed, not in the fridge. Symbolic image.

Related

Related compounds

Same compounds: all in the TPD lexicon.

Test/Tren/EQ Mix 400
Testosterone Enanthate Component, single entry in the lexicon Equipoise (Boldenone Undecylenate) Component, single entry in the lexicon Trenbolone Enanthate Component, single entry in the lexicon Test/EQ Mix 400 Without trenbolone Test/Tren Long 300 Without boldenone Test/Tren Short 150 Testosterone and trenbolone with short esters Tren/Test/Mast Short 150 Three short esters with drostanolone

FAQ

Common questions about Test/Tren/EQ Mix 400

Short answers, each backed by the sources below.

What is in Test/Tren/EQ Mix 400?

Testosterone enanthate, boldenone undecylenate and trenbolone enanthate in a 3 to 3 to 2 ratio, ready to use in oil. All three esters are long. Trenbolone is the smallest share but according to the lexicon it sets the side effect profile. Because the shares are fixed, only the total amount can be changed.111213

Are there studies on this blend?

No. As of 26 October 2026 ClinicalTrials.gov lists no study on the blend, none on trenbolone and none on boldenone. For testosterone enanthate by injection it lists 9 phase 1, 13 phase 2, 7 phase 3 and 3 phase 4 trials.10

What is special about trenbolone?

According to a review trenbolone binds the androgen receptor about three times as strongly as testosterone. In tissue testosterone is partly converted into DHT and estradiol while trenbolone is converted into weaker androgens. That is why it acts less androgenic and less estrogenic in tissue. In various animal species it increased muscle and bone mass and lowered body fat. There are no controlled trials in humans.2

What do studies on the testosterone share show?

In a randomised trial with 43 healthy men testosterone enanthate increased muscle size and strength, most of all with strength training: 22 kg in the bench press, 38 kg in the squat and 6.1 kg of fat-free mass in 10 weeks. In another trial haemoglobin and haematocrit rose linearly with the amount, more in older men.13

Which side effects do users report?

The HAARLEM study followed 100 men through a self-chosen cycle and one year. All reported at least one side effect, most often fluid retention (56 percent) and agitation (36 percent) during the cycle and lower libido afterwards (58 percent). Acne occurred in 28 percent and breast tissue growth in 19 percent. Four had a serious event. After one year the observed effects were back at baseline. Effects typical of trenbolone such as sleep problems or prolactin effects were not reported separately there.5

What happens to heart and blood lipids?

In strength athletes on several steroids HDL cholesterol fell from 1.08 to 0.43 mmol/l and had not recovered six weeks after stopping. Long-term users pumped less blood per heartbeat (ejection fraction 52 vs 63 percent) and had more coronary plaque. A review of 49 studies also describes partly higher blood pressure and a thickened heart wall.674

What happens to the body's own hormone production?

Testosterone from outside suppresses the hormone axis. After one injection of testosterone enanthate LH and FSH stayed suppressed as long as the level was raised. In the WHO study with weekly injections a cumulative 65 percent had no detectable sperm after 6 months. After stopping it took a median of 3.7 months until at least 20 million sperm per ml returned and 6.7 months until baseline. How long recovery takes after this blend has not been studied.89

Glossary

Terms explained briefly

Dotted words in the text open these explanations directly.

19-nor steroid
Testosterone derivative without the carbon atom at position 19. Nandrolone and trenbolone belong to this group.
Androgen receptor
Protein inside the cell that testosterone and related compounds bind to. Genes are then read, for example for muscle building.
Cohort study
Observational study that follows a group over time. Participants choose compounds and amounts themselves, there is no random allocation.
Ester
An attached fatty acid chain. It makes the compound oil-soluble and sets how slowly it is released from the depot. It is split off in the blood.
Estradiol
The main oestrogen. In men it is also made from testosterone and rises with the testosterone level.
Haematocrit
Share of red blood cells in the blood. If it rises a lot, the blood gets thicker.
HDL cholesterol
The so-called good cholesterol. It carries fat from the vessels back to the liver.
Hormone axis (HPTA)
Hypothalamus, pituitary and testes together control the body's own testosterone production. Testosterone from outside suppresses this axis.
Progesterone receptor
Receptor for the hormone progesterone. Some anabolic steroids also act here, not only at the androgen receptor.
Prolactin
Pituitary hormone. If it is raised, libido and erection can suffer.
Pumping function (ejection fraction)
Share of blood the left ventricle pushes out with each beat. A lower value means weaker pumping.
Supraphysiologic
Above the natural range the body makes on its own.

Practice

PCT / after coming off

Anabolic substances suppress the body's own testosterone production in the research context. Anyone who comes off a cycle (rather than running it continuously) typically uses a post-cycle therapy (PCT) to restart the natural axis (HPTA).

The standard scheme in the research/literature context combines a SERM (tamoxifen and/or clomifene, alternatively enclomiphene) over roughly four weeks, optionally preceded by HCG to re-sensitise the testes before the SERM block. An aromatase inhibitor (anastrozole/exemestane) belongs more to on-cycle estrogen control than to the PCT itself.

Tip: order the PCT items together with the cycle so they are already on hand when you come off.

This information serves research and educational purposes only. Not medical advice.

Tamoxifen (Nolvadex) SERM, core of PCT View Clomid (Clomifen) SERM, core of PCT View Enclomiphene (Androxal) SERM alternative View HCG testicular restart before PCT View Arimidex (Anastrozole) aromatase inhibitor, on-cycle View Aromasin (Exemestane) aromatase inhibitor, on-cycle View

Evidence

Sources

Show all 13 sourcesHide sources 9 Studies1 Trial registry3 Database checked 26 Oct 2026

Studies

  1. Supraphysiologic testosterone doses, muscle size and strength in healthy men

    Bhasin et al., New England Journal of Medicine, 1996

    DOI 10.1056/NEJM199607043350101 PMID 8637535
    Checked 26 Oct 2026 via doi.org, Crossref, PubMed
  2. Review on trenbolone: binding, metabolism and tissues

    Yarrow et al., Steroids, 2010

    DOI 10.1016/j.steroids.2010.01.019 PMID 20138077
    Checked 26 Oct 2026 via doi.org, Crossref, PubMed
  3. Red blood cell production (haemoglobin, haematocrit) by testosterone dose

    Coviello et al., Journal of Clinical Endocrinology and Metabolism, 2008

    DOI 10.1210/jc.2007-1692 PMID 18160461
    Checked 26 Oct 2026 via doi.org, Crossref, PubMed
  4. Review: heart and metabolism under anabolic steroids, 49 studies

    Achar et al., American Journal of Cardiology, 2010

    DOI 10.1016/j.amjcard.2010.05.013 PMID 20816133
    Checked 26 Oct 2026 via doi.org, Crossref, PubMed
  5. HAARLEM study: 100 users followed for one year

    Smit et al., Scandinavian Journal of Medicine and Science in Sports, 2020

    DOI 10.1111/sms.13843 PMID 33038020
    Checked 26 Oct 2026 via doi.org, Crossref, PubMed (Heft 2021, online 2020)
  6. Blood lipids under anabolic steroids in strength athletes

    Hartgens et al., British Journal of Sports Medicine, 2004

    DOI 10.1136/bjsm.2003.000199 PMID 15155420
    Checked 26 Oct 2026 via doi.org, Crossref, PubMed
  7. Heart function and coronary plaque after long-term use

    Baggish et al., Circulation, 2017

    DOI 10.1161/CIRCULATIONAHA.116.026945 PMID 28533317
    Checked 26 Oct 2026 via doi.org, Crossref, PubMed
  8. Level over time after one injection of testosterone enanthate

    Schürmeyer and Nieschlag, International Journal of Andrology, 1984

    DOI 10.1111/j.1365-2605.1984.tb00775.x PMID 6434435
    Checked 26 Oct 2026 via doi.org, Crossref, PubMed
  9. WHO study: sperm production under weekly injections

    WHO Task Force on Methods for the Regulation of Male Fertility, The Lancet, 1990

    DOI 10.1016/0140-6736(90)92416-F PMID 1977002
    Checked 26 Oct 2026 via doi.org, Crossref, PubMed (Titel und Seiten, Datensatz ohne DOI)

Trial registry

  1. Trials on testosterone enanthate on ClinicalTrials.gov

    ClinicalTrials.gov, U.S. National Library of Medicine

    Checked 26 Oct 2026 via ClinicalTrials.gov API v2

Database

  1. Testosterone enanthate in ChEMBL: mechanism and approval

    ChEMBL, EMBL-EBI (CHEMBL1200335)

    Checked 26 Oct 2026 via ChEMBL
  2. Boldenone undecylenate in ChEMBL: not approved, no recorded mechanism

    ChEMBL, EMBL-EBI (CHEMBL2106059)

    Checked 26 Oct 2026 via ChEMBL
  3. Trenbolone acetate in ChEMBL: not approved, no recorded mechanism

    ChEMBL, EMBL-EBI (CHEMBL1698011)

    Checked 26 Oct 2026 via ChEMBL

In the shop

Test/Tren/EQ Mix 400 in the catalog

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