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Muscle & performance

Test/EQ Mix 400

Testosterone enanthate and boldenone undecylenate at 200mg per ml each: a slow blend of two long esters where hematocrit and bloodwork set the pace.

4 min read 12 sources

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Glass vial of Test/EQ Mix 400 with TPD label on a white podium with a light blue ring of light in front of a bright lab background, symbolic image
No study of its own · not approved

Summary

At a glance

Sources checked on 26 Oct 2026

Phase 3 on testosterone enanthate
7 trials

on the registry as injection, none on the blend or on boldenone, as of 26 Oct 202610

Fat-free mass in 10 weeks
+6.1 kg

Testosterone enanthate with strength training, randomised trial with 43 healthy men1

Back to baseline
9 days

After one injection of testosterone enanthate: peak level at 8 to 24 hours6

HDL cholesterol
-60.2 %

Strength athletes, several steroids at once over 8 or 14 weeks, not recovered after a 6-week break4

With high blood pressure
51 %

Current users on 24-hour monitoring, compared with 17 percent without steroids5

  • Test/EQ Mix 400 is a ready-made oil of testosterone enanthate and boldenone undecylenate in equal parts. Both esters are long, testosterone enanthate is released from the oil over days.6
  • There is no study on the blend and no controlled human study on boldenone. All figures here come from studies on testosterone alone or from observations in users.10
  • In trials testosterone enanthate increased fat-free mass, muscle size and strength, most of all with training and depending on the amount. Haemoglobin and haematocrit rose along with it.123
  • In strength athletes on several steroids HDL cholesterol fell by about 60 percent. Current users had high blood pressure more often, current and former users a stiffer aorta.45
  • The body's own hormone axis is suppressed. According to ChEMBL testosterone enanthate has been approved since 1953, boldenone undecylenate is not. Everything here is the state of research, not a recommendation.61112
Profile

Updated October 2026

Class
Anabolic steroid

oil blend of two esters

Contents
200mg testosterone enanthate + 200mg boldenone undecylenate per ml (400mg/ml)
Use
Intramuscular, ready-to-use oil

10 ml

Frequency
2x per week
WADA
Banned

category S1

Sizes
10 ml

1 size in the shop

Contents 14 sections
  1. At a glance
  2. What is Test/EQ Mix 400?
  3. How it works
  4. Use and dosing
  5. Injection
  6. What the research shows
  7. Research status
  8. Side effects
  9. Storage
  10. PCT / after coming off
  11. Related compounds
  12. FAQ
  13. Glossary
  14. Sources

What is Test/EQ Mix 400?

Test/EQ Mix 400 is a ready-made oil solution of two compounds: 200mg testosterone enanthate and 200mg boldenone undecylenate (Equipoise, EQ for short) per ml, 400mg/ml in total. One vial holds 10 ml and needs no mixing. Both esters are long, so the blend is built for few injections per week and steady levels.

Testosterone is the body's main androgen. Boldenone is a testosterone derivative with one extra double bond that comes from veterinary medicine and was never approved for humans. In a research context the combination is seen as a slow building blend with its own testosterone base.

This information is for research and educational purposes only. No medical advice.

Comparison

One, two or three compounds

Which compounds each oil contains, as stated in the TPD lexicon. Tap a card to open its entry.

1 compound Testosterone Enanthate Testosterone Boldenone Trenbolone 1 of 3 compounds 1 compound Equipoise (Boldenone Undecylenate) Testosterone Boldenone Trenbolone 1 of 3 compounds
2 compounds Test/EQ Mix 400 Testosterone Boldenone Trenbolone 2 of 3 compounds
3 compounds Test/Tren/EQ Mix 400 Testosterone Boldenone Trenbolone 3 of 3 compounds

Simplified: the same compounds do not mean the same amount per ml. Every extra compound brings its own side effects.

How it works

Both compounds bind the androgen receptor in muscle, where they drive protein synthesis, nitrogen balance and strength. Testosterone is partly aromatized to estradiol and partly converted to DHT. Boldenone aromatizes less and is somewhat milder on the androgenic side.

Boldenone is also known for stimulating red blood cell production. Together with the testosterone share this can push hematocrit up markedly. The undecylenate ester is very long (half-life around two weeks) while enanthate sits at about 4 to 5 days, so the full effect builds over several weeks.

Where it acts

One receptor, several organs

Tap a point or a card. Grey = effect seen in trials.

  1. Hypothalamus and pituitary

    LH and FSH suppressed as long as testosterone was raised6

    Observed in trials
  2. Testes

    Sperm production suppressed: no sperm in 65 percent after 6 months, recovery within months after stopping7

    Observed in trials
  3. Blood

    Haemoglobin and haematocrit rose with the testosterone dose, more in older men3

    Observed in trials
  4. Liver and blood lipids

    HDL cholesterol fell by about 60 percent under several anabolic steroids at once4

    Observed in trials
  5. Heart and vessels

    24-hour blood pressure higher (systolic 132 vs 124 mmHg), aorta stiffer5

    Observed in trials
  6. Skeletal muscle

    Muscle size in arms and legs and strength increased, most with training1

    Observed in trials
  7. Skin

    Acne in 28 of 100 users, breast tissue growth in 198

    Observed in trials

Targets in the ChEMBL database11

Compound CHEMBL1200335: one recorded mechanism, agonist with direct binding to the human receptor.

Androgen receptor
Gene
AR
UniProt
P10275
ChEMBL
CHEMBL1871
Action
Agonist, direct binding
Translucent glass muscle fibres with docked blue steroid molecules, next to them a growing cluster of smooth disc-shaped cells
Testosterone docks at the androgen receptor in muscle, red blood cell production rises as well. Symbolic image.

Use and dosing

Dosing

Dosing, titration and syringe

Loading the dosing information …

  • Getting started and stepping up, drawn as a staircase
  • Units on the U-100 syringe for every step
  • How long one vial lasts
Glass vial of Test/EQ Mix 400 with clear, slightly yellowish oil and a grey stopper, next to it a closed syringe with its cap on a light table
Ready-to-use oil, no mixing needed. Symbolic image.

Injection

The blend is given intramuscularly, usually into the glute, thigh or shoulder. Use a thicker needle to draw up and a thin one (23G to 25G) to inject. Disinfect the stopper and the site first. Rotate sites regularly so tissue does not harden.

Because both esters are long, subcutaneous injection into belly or thigh fat is possible in principle, with a short needle and no more than about 0.5ml per site. If redness, pain or lumps appear, go back to intramuscular injection.

What the research shows

For testosterone it is well documented that muscle mass and strength rise with the dose: supraphysiologic doses over ten weeks increased muscle size and strength in healthy men (Bhasin, 1996). In a dose-response study fat-free mass and strength grew with every step while HDL fell in step (Bhasin, 2001). The same data show hemoglobin and hematocrit rising linearly with the dose (Coviello, 2008).

There are no controlled human studies on boldenone; experience comes from veterinary use and observations in users. No study exists for the fixed combination, so expectations are derived from the single compounds. Research in strength athletes also shows that steroid cycles cut HDL sharply (Hartgens, 2004) and go along with lastingly higher blood pressure (Rasmussen, 2018).

Study results

What the trials measured

Interactive: switch tabs, tap points, show or hide arms. Arms are named relative to each other, no dosing advice.

Strength gain in 10 weeks1

Healthy men, weekly injection of testosterone enanthate or placebo, gain in kg

Bench press1 43 men, 10 weeks
Placebo without training -1 kg
Testosterone without training +9 kg
Testosterone with training +22 kg
Squat1 43 men, 10 weeks
Placebo without training +3 kg
Testosterone without training +16 kg
Testosterone with training +38 kg

Trial with testosterone enanthate alone, not with the blend. The abstract gives no figures for the placebo with training group. Mood and behaviour did not change in any group.

Blood lipids under several anabolic steroids4

Change in percent, 19 strength athletes, self-chosen cycle over 8 or 14 weeks

HDL cholesterol4
Several anabolic steroids at once -60.2%
Apolipoprotein A14
Several anabolic steroids at once -49.6%
Apolipoprotein B4
Several anabolic steroids at once +37.5%
Lipoprotein (a)4
Several anabolic steroids at once -83.1%

Percentages calculated from the mean values in the abstract: HDL from 1.08 to 0.43 mmol/l, apo-A1 from 1.41 to 0.71 g/l, apo-B from 0.96 to 1.32 g/l, Lp(a) from 189 to 32 U/l. Six weeks after stopping the values had not yet recovered. Nandrolone decanoate alone did not change blood lipids in the second part of the study.

High blood pressure in users5

Share with systolic hypertension on 24-hour monitoring, strength athletes

Cross-sectional study, 100 men5 37 current users, 30 controls
Current users 51%
Strength athletes without steroids 17%

Systolic 24-hour average 132 vs 124 mmHg. Aortic distensibility was 21 percent lower in current and in former users than in controls, so the aorta was stiffer.

Calm study room in window light, on the desk a small frosted glass muscle sculpture and a stack of reddish glass discs shaped like blood cells
Studies exist on testosterone alone, not on the blend. Symbolic image.

Research status

Where the research stands

There is no study on the blend and the registry finds none on boldenone undecylenate. Shown are registered trials with testosterone enanthate given by injection. As of 26 Oct 2026. 10

  1. Preclinical How anabolic steroids act in animals and cells (review)
  2. Phase 1 9 trials on components, 8 completed, 1 stopped early
  3. Phase 2 13 trials on components, 8 completed, 1 stopped early
  4. Phase 3 7 trials on components, 6 completed
  5. Approval not yet
586 participants in phase 3 Planned figures for running trials Not approved as a blend. According to ChEMBL testosterone enanthate has been approved since 1953, boldenone undecylenate is not approved.11
Show all 23 phase 2, 3 and 4 trials on a timelineHide timeline 17 completed 5 running 1 stopped early 0 with results
Phase 2
Phase 3
Phase 4

Bar: start to primary endpoint (planned for running trials). Green = completed, blue = progress until 26 Oct 2026, grey = stopped early. Phase 1: 9 trials on safety and pharmacokinetics, not shown as bars.

Side effects

  • Blood count: hematocrit and hemoglobin rise, often more with the boldenone share than with testosterone alone. Thicker blood raises the risk of thrombosis, so have hematocrit checked before, during and after a cycle.
  • Lipids and blood pressure: HDL drops, apolipoprotein B rises and blood pressure can climb for good. Measure regularly rather than guess.
  • Estrogen: testosterone aromatizes, boldenone less so. Water retention and sore nipples up to gynecomastia are possible, so check estradiol in the bloodwork.
  • Hormone axis: your own testosterone production is suppressed, testicle size and fertility drop. After the cycle the axis needs weeks to months.
  • Other: acne, oily skin, hair loss if predisposed, restlessness and a clearly increased appetite that is typical for boldenone.

Heart and vessels carry the load over years, which is why blood pressure, lipids and hematocrit belong to every cycle.

Tolerability

Frequency in the HAARLEM study

Out of 100 users, how many had the event. One cohort without a comparison group, participants chose compounds and amounts themselves.

100 of 100 All 100 users
Event All 100 users
At least one side effect8 100%
Lower libido after the cycle8 58%
Fluid retention during the cycle8 56%
Agitation during the cycle8 36%
Acne8 28%
Breast tissue growth (gynecomastia)8 19%

Four participants had a serious event: heart failure, pancreatitis, suicidal thoughts and a flare of ulcerative colitis. One year after the start the observed effects were back at baseline.8

Storage

Store at room temperature, in the dark and in the original packaging, out of reach of children. Do not put it in the fridge, because cold makes the compounds crystallize out of the oil. If you see crystals, warm the vial in your hand or in warm water and swirl until the solution is clear again. Disinfect the stopper before every draw.

Four glass vials with golden oil and grey caps on a white shelf, next to them a folded light blue cloth
Store at room temperature, dark and closed, not in the fridge. Symbolic image.

Related

Related compounds

Same compounds: all in the TPD lexicon.

Test/EQ Mix 400
Testosterone Enanthate Component, single entry in the lexicon Equipoise (Boldenone Undecylenate) Component, single entry in the lexicon Boldenone Cypionate Boldenone with a shorter ester Test/Tren/EQ Mix 400 Same base plus trenbolone enanthate Test/Deca Mix 500 Testosterone enanthate plus nandrolone decanoate Test/Tren Long 300 Testosterone enanthate plus trenbolone enanthate Testosterone Cypionate Another long testosterone ester

FAQ

Common questions about Test/EQ Mix 400

Short answers, each backed by the sources below.

What is in Test/EQ Mix 400?

A ready-made oil with testosterone enanthate and boldenone undecylenate in equal parts. Boldenone is a testosterone derivative with one extra double bond. Both esters are long and the oil needs no mixing. Because the shares are fixed, any change in amount always changes both compounds.1112

Are there studies on this blend?

No. As of 26 October 2026 ClinicalTrials.gov lists no study on the blend and none on boldenone. For testosterone enanthate by injection it lists 9 phase 1, 13 phase 2, 7 phase 3 and 3 phase 4 trials. Everything here therefore comes from studies on testosterone alone or from observations in users.10

What do studies on the testosterone share show?

In the best-known trial 43 healthy men received testosterone enanthate or placebo once a week for 10 weeks, with or without strength training. Without training bench-press strength rose by 9 kg on testosterone and fell by 1 kg on placebo. With training they gained 22 kg in the bench press, 38 kg in the squat and 6.1 kg of fat-free mass. Mood and behaviour did not change in any group. Testosterone alone was tested, not the blend.1

Why does haematocrit matter so much?

Testosterone stimulates red blood cell production. In a trial with five dose levels haemoglobin and haematocrit rose linearly with the amount, more in older men than in young men. Thicker blood puts strain on the circulation. For boldenone there is no controlled human study on this effect, the lexicon treats it as an extra driver.3

What happens to blood lipids and blood pressure?

In strength athletes who took several steroids at once for 8 or 14 weeks HDL cholesterol fell from 1.08 to 0.43 mmol/l while apolipoprotein B rose. Six weeks after stopping the values had not yet recovered, more slowly after the longer cycle. In another study current users had high blood pressure more often (51 vs 17 percent) and like former users a stiffer aorta.45

What happens to the body's own hormone production?

Testosterone from outside suppresses the hormone axis. After one injection of testosterone enanthate LH and FSH stayed suppressed as long as the level was raised. In the WHO study with weekly injections a cumulative 65 percent had no detectable sperm after 6 months. After stopping it took a median of 3.7 months until at least 20 million sperm per ml returned and 6.7 months until baseline. How long recovery takes after this blend has not been studied.67

How long does one injection last?

Measured for testosterone enanthate: levels peaked 8 to 24 hours after the injection at four to five times the starting level and were back at baseline after 9 days. For boldenone undecylenate the sources checked here contain no measurement in humans.6

Glossary

Terms explained briefly

Dotted words in the text open these explanations directly.

Androgen receptor
Protein inside the cell that testosterone and related compounds bind to. Genes are then read, for example for muscle building.
Apolipoproteins
Proteins that carry blood fats. Apo-A1 belongs to HDL, apo-B to the particles that carry fat into the vessels.
Aromatisation
The enzyme aromatase converts part of the testosterone into estradiol. This is where water retention and breast tissue effects come from.
Azoospermia
No sperm detectable in the ejaculate.
Cohort study
Observational study that follows a group over time. Participants choose compounds and amounts themselves, there is no random allocation.
Depot
The oil solution stays in the tissue after the injection and releases the compound over days.
Ester
An attached fatty acid chain. It makes the compound oil-soluble and sets how slowly it is released from the depot. It is split off in the blood.
Fat-free mass
Body weight without fat: muscle, bone, organs and water.
Haematocrit
Share of red blood cells in the blood. If it rises a lot, the blood gets thicker.
HDL cholesterol
The so-called good cholesterol. It carries fat from the vessels back to the liver.
Hormone axis (HPTA)
Hypothalamus, pituitary and testes together control the body's own testosterone production. Testosterone from outside suppresses this axis.
LH and FSH
Pituitary hormones. LH drives testosterone production in the testes, FSH drives sperm production.

Practice

PCT / after coming off

Anabolic substances suppress the body's own testosterone production in the research context. Anyone who comes off a cycle (rather than running it continuously) typically uses a post-cycle therapy (PCT) to restart the natural axis (HPTA).

The standard scheme in the research/literature context combines a SERM (tamoxifen and/or clomifene, alternatively enclomiphene) over roughly four weeks, optionally preceded by HCG to re-sensitise the testes before the SERM block. An aromatase inhibitor (anastrozole/exemestane) belongs more to on-cycle estrogen control than to the PCT itself.

Tip: order the PCT items together with the cycle so they are already on hand when you come off.

This information serves research and educational purposes only. Not medical advice.

Tamoxifen (Nolvadex) SERM, core of PCT View Clomid (Clomifen) SERM, core of PCT View Enclomiphene (Androxal) SERM alternative View HCG testicular restart before PCT View Arimidex (Anastrozole) aromatase inhibitor, on-cycle View Aromasin (Exemestane) aromatase inhibitor, on-cycle View

Evidence

Sources

Show all 12 sourcesHide sources 9 Studies1 Trial registry2 Database checked 26 Oct 2026

Studies

  1. Supraphysiologic testosterone doses, muscle size and strength in healthy men

    Bhasin et al., New England Journal of Medicine, 1996

    DOI 10.1056/NEJM199607043350101 PMID 8637535
    Checked 26 Oct 2026 via doi.org, Crossref, PubMed
  2. Dose response in young men, five levels over 20 weeks

    Bhasin et al., American Journal of Physiology: Endocrinology and Metabolism, 2001

    DOI 10.1152/ajpendo.2001.281.6.E1172 PMID 11701431
    Checked 26 Oct 2026 via doi.org, Crossref, PubMed
  3. Red blood cell production (haemoglobin, haematocrit) by testosterone dose

    Coviello et al., Journal of Clinical Endocrinology and Metabolism, 2008

    DOI 10.1210/jc.2007-1692 PMID 18160461
    Checked 26 Oct 2026 via doi.org, Crossref, PubMed
  4. Blood lipids under anabolic steroids in strength athletes

    Hartgens et al., British Journal of Sports Medicine, 2004

    DOI 10.1136/bjsm.2003.000199 PMID 15155420
    Checked 26 Oct 2026 via doi.org, Crossref, PubMed
  5. Blood pressure and aortic stiffness in current and former users

    Rasmussen et al., Journal of Hypertension, 2018

    DOI 10.1097/hjh.0000000000001546 PMID 28863033
    Checked 26 Oct 2026 via doi.org, Crossref, PubMed
  6. Level over time after one injection of testosterone enanthate

    Schürmeyer and Nieschlag, International Journal of Andrology, 1984

    DOI 10.1111/j.1365-2605.1984.tb00775.x PMID 6434435
    Checked 26 Oct 2026 via doi.org, Crossref, PubMed
  7. WHO study: sperm production under weekly injections

    WHO Task Force on Methods for the Regulation of Male Fertility, The Lancet, 1990

    DOI 10.1016/0140-6736(90)92416-F PMID 1977002
    Checked 26 Oct 2026 via doi.org, Crossref, PubMed (Titel und Seiten, Datensatz ohne DOI)
  8. HAARLEM study: 100 users followed for one year

    Smit et al., Scandinavian Journal of Medicine and Science in Sports, 2020

    DOI 10.1111/sms.13843 PMID 33038020
    Checked 26 Oct 2026 via doi.org, Crossref, PubMed (Heft 2021, online 2020)
  9. Pharmacology of anabolic steroids (review)

    Kicman, British Journal of Pharmacology, 2008

    DOI 10.1038/bjp.2008.165 PMID 18500378
    Checked 26 Oct 2026 via doi.org, Crossref, PubMed

Trial registry

  1. Trials on testosterone enanthate on ClinicalTrials.gov

    ClinicalTrials.gov, U.S. National Library of Medicine

    Checked 26 Oct 2026 via ClinicalTrials.gov API v2

Database

  1. Testosterone enanthate in ChEMBL: mechanism and approval

    ChEMBL, EMBL-EBI (CHEMBL1200335)

    Checked 26 Oct 2026 via ChEMBL
  2. Boldenone undecylenate in ChEMBL: not approved, no recorded mechanism

    ChEMBL, EMBL-EBI (CHEMBL2106059)

    Checked 26 Oct 2026 via ChEMBL

In the shop

Test/EQ Mix 400 in the catalog

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